Measuring Color Absorption in Biopharmaceuticals: How Spectrophotometers Determine Analyte Concentrations

The world of biopharmaceuticals is continually expanding and changing. In the laboratory, new formulations are leading to new medications that help patients achieve a better quality of life. Breakthroughs in analytical methods now utilize color absorption values with advanced spectrophotometry for the determination of analyte concentrations. These methods have made quantifying highly accurate and offer a quick and easy method of biopharmaceutical development.

color absorption
In the laboratory, advanced spectrophotometers can measure color absorption values to determine analyte concentrations in biopharmaceutical research and development.
Image Source: Flickr user University of Michigan School of Natural Resources & Environment

Biopharmaceutical manufacturers depend on reliable analysis of analyte concentrations to quantify the various components of medications. Traditional methods of analysis relied on dilution measurements to determine the analyte concentration in a sample solution, but these were time-consuming and left too much room for error. Spectrophotometry revolutionized the biopharmaceutical market with the advent of color absorption measurement. Measuring color absorption provided a reliable method in the determination of many various analyte concentrations. This method is non-destructive and can accurately determine the various components of a sample. By using advanced technology and precise mathematical formulas, spectrophotometry quickly revolutionized the world of biopharmaceutical research and development.

The purpose of color absorption technology

Measuring analyte concentrations covers a variety of applications in the field of biopharmaceuticals. From glucose concentration to protein analysis, spectrophotometric technology provides an ideal method to obtain accurate measurements. In recent years, biopharmaceutical proteins have skyrocketed and new products are quickly flooding the market. Biopharmaceutical companies must rely on the latest technology of analysis in order to meet the market demands. UV Spectrophotometry offers an affordable alternative to the various analytical methods available, yet still provides accurate and consistent results.

antibody protein color absorption
Antibody proteins are commonly used in biopharmaceuticals and require accurate quantification of concentration levels for safe and effective dosage measurements. Image Source: Flickr’ user Anthony D’Onofrio

UV Spectrophotometers are also highly effective in analyzing drug-to-antibody ratios (DAR). According to an article published by the NCBI (National Center for Biotechnology Information), “UV/Vis spectroscopy is a simple and convenient method to determine protein concentrations as well as the average number of drugs that are conjugated to the antibody in an antibody-drug conjugate (ADC). Using the measured absorbances of the ADC and the extinction coefficients of the antibody and the drug, the average drug-to-antibody ratio (DAR) can be determined.”

The benefits of spectral analysis continue to grow, and more methods and uses for color absorption values continue to transform the science of biopharmaceutical research and development. In addition to effectively determining analyte concentrations, UV spectroscopy also aids in the detection of impurities that can be found in prescription drugs. Color absorption levels can be used to differentiate various compounds and substances, and spectral analysis considered a reliable method of analysis by the FDA (food and drug administration). The data provided is necessary to meet these stringent regulations and assures both quality and safety of the product.

pharmaceutical capsule color absorption
UV Spectrophotometry is used for a variety of applications in pharmaceuticals and assures both the quality and safety of prescription medications.
Image Source: Flickr user Oliver.Dodd

UV Spectrophotometers

Spectrophotometers are designed to meet various analytical needs and are available in a variety of shapes and sizes. Each design format is unique to its application and typically caters to the needs and challenges of the industry. For biopharmaceutical research and manufacturing, using the right instrumentation is critical. The right tools coupled with the knowledge and support from color absorption and color measurement experts leads to better products and more efficient use of the instrumentation. At HunterLab we specialize in the development and research of pharmaceutical products. Our support team is dedicated to helping you make the most of your investment and achieve the results you want. Contact HunterLab today to learn more about color absorption and spectrophotometry in biopharmaceuticals.

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